This past weekend, I ran a 1 tank with ethanol fuel, followed by a tank without ethanol. Two days later, I ran a tank without ethanol, followed by a tank with ethanol. This was on the same stretch of road. The non-ethanol fuel cost $0.31 more per gallon. Although the engine temp remained the same, the thermostat opened about 200 more times on the ethanol fuel, than on the non-ethanol fuel. I used .7 gallons less fuel with the non-ethanol fuel. The fuel runs were 296 miles with the ethanol, and 291 miles with the non-ethanol fuel, just because of where the stations were. I'd say that's a reasonable sample distance. All 4 runs were with the AC on. I got 4 MPG better fuel economy with the non-ethanol fuel.
Over the last several weeks, I have run 7 tanks of ethanol fuel, with the AC on, and got about 31 MPG, each tank. I ran 285 or more miles per tank. That is consistent, and should serve as a baseline.